|
KMID : 0613820180280111301
|
|
Journal of Life Science
2018 Volume.28 No. 11 p.1301 ~ p.1315
|
|
|
Mytilin B, an Antimicrobial Peptide from the Hemocyte of the Hard-shelled Mussel, Mytilus coruscus: Isolation, Purification, and Characterization
|
|
Lee Min-Jeong
Oh Ryun-Kyoung
Kim Young-Ok
Nam Bo-Hye
Kong Hee-Jeong
Kim Joo-Won
Park Jung-Youn
Seo Jung-Kil
Kim Dong-Gyun
|
|
|
Abstract
|
|
|
|
We purified an antimicrobial peptide from the acidified hemocyte extract of Mytilus coruscus by C18 reversed-phase high-performance liquid chromatography (RP-HPLC). The peptide was 4041.866 Da based on matrix-assisted laser desorption ionization time-of-flight mass spectrophotometer (MALDI- TOF/MS) and the 25 amino acids of the N-terminus sequence were identified. Comparison of this sequence of the purified peptide with the N-terminus sequences of other antimicrobial peptides revealed 100% identity with the mytilin B precursor of Mytilus coruscus. We also identified a 312 bp open-reading frame (ORF) encoding 103 amino acids based on the obtained amino acid residues. The nucleotide sequence of this ORF and the amino acid sequence also revealed 100% identity with the mytilin B precursor of Mytilus coruscus. We synthesized two antimicrobial peptides with an alanine residue in the C-terminus, and designated them mytilin B1 and B2. These two antimicrobial peptides showed antimicrobial activity against gram-positive bacteria, including Bacillus cereus and Streptococcus parauberis (minimal effective concentration, MECs 41.6-89.7 μg/ml), gram-negative bacteria, including Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia stuartii, Pseudomonas aeruginosa, and Vibrio ichthyoenteri (MECs 7.4-39.5 μg/ml), and the fungus Candida albicans (MECs 26.0-31.8 μg/ml). This antimicrobial activity was stable under heat and salt conditions. Furthermore, the peptides did not exhibit significant hemolytic activity or cytotoxic effects. These results suggest that mytilin B could be applied as alternative antibiotic agent, and they add to the understanding of the innate immunity of hard-shelled mussels.
|
|
|
KEYWORD
|
|
|
Antimicrobial peptide, innate immunity, mytilin B, Mytilus coruscus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|